Better Brewing with the Oculyze BB 2.0


 

Better Brewing with the Oculyze BB 2.0

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Oculyze BB user manual
& training videos

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Access the
webapp login

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Access the Quick guide for
Oculyze Microscope Digitalization

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Oculyze BB user manual
& training videos

Click here

Access the
webapp login

Click here

Access the Quick guide for
Oculyze Microscope Digitalization

Click here

Comparing our device to other systems:


 

The VLB Berlin was commissioned as an external and independent institute for a system validation to demonstrate the correct functionality of the Oculyze counting system. The following analysis were performed:


  • Determination of cell concentration and viability of different brewer’s yeast strains using the following methods:

    – Oculyze System
    – Manual evaluation with the hemocytometer at a professional microscope by staff with appropriate expertise and several years of experience
    – Another automated system, that is designed for the determination of the cell concentration and the live/dead ratio of brewer’s yeast: NucleoCounter YC-100

  • Analysis of at least three different brewer’s yeast strains; each in three different batches

  • Analysis of all yeast strains and batches in watery solution and wort. Measurements within wort where done to evaluate the influence of turbid particles on the analysis of cell concentration.

  • Each batch of the yeast populations was analysed with the respective systems three times. The average of the 3 counts was compared with the average of the other devices.

 
 
IMPORTANT!

An internal validation is a great way to gain confidence and experience with new lab equipment. However for it to have any value it needs to be done according to a minimum of scientific standards.

If you are considering doing a similar comparison please read the following to know how they tested it.

You can read the full validation here.
 
A few important things to note when doing a similar test comparison:

1) To make a statistically valid analysis each sample must be analysed with each system at least three times
2) Make sure you are treating each sample in the same way. Points to consider:
→ at what point you're splitting the sample
→ when and where was the sample taken from
→ what are the incubation times (when testing with methylene blue - after 15 mins in a methylene blue solution the cells start to die, so you have to do the tests in parallel)
→ always empty the chamber and clean it
→ make sure the sample is homogeneous (shake it)
→ if you're using a hemocytometer - make sure the numbers of squares is consistent with the oculyze system - one photo taken with the oculyze system is roughly equal to one 0,2 x 0,2 mm square (located in the central square) on the hemocytometer
→ The automatic counters will document the result. Make sure you document your counting results AND how you obtained them. Document the images you are counting under the microscope. Otherwise you will assume you counted 100% correct with no means of checking if this is true. This is specially critical when checking for viability as here opinions and counts tend to differ. Ocular cameras can be obtained for less than 100 USD at Amazon or for example here.

If you have any questions during the comparison process - please consult our team of experts at support@oculyze.de