Better Brewing with the Oculyze BB 2.0


 

Better Brewing with the Oculyze BB 2.0

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Comparing our device to other systems:


 

The VLB Berlin was commissioned as an external and independent institute for a system validation to demonstrate the correct functionality of the Oculyze counting system. The following analysis were performed:


  • Determination of cell concentration and viability of different brewer’s yeast strains using the following methods:

    – Oculyze System
    – Manual evaluation with the Thoma chamber at a microscope by staff with appropriate expertise
    – Another automated system, that is designed for the determination of the cell concentration and the live/dead ratio of brewer’s yeast: NucleoCounter YC-100

  • Analysis of at least three different brewer’s yeast strains; each in three different batches

  • Analysis of all yeast strains and batches in watery solution and wort. Measurements within wort where done to evaluate the influence of turbid particles on the analysis of cell concentration.

  • Each batch of the yeast populations was analysed with the respective systems three times.

 
 
IMPORTANT!
If you are considering doing a similar comparison please read the following to know how they tested it.
You can read the full validation here .
Or read the publication here.
A few important things to note when doing a similar test comparison:

1) To make a statistically valid analysis each sample must be analysed with each system at least three times
2) Make sure you are treating each sample in the same way. Points to consider:
→ at what point you're splitting the sample
→ when and where was the sample taken from
→ what are the incubation times (when testing with methylene blue - after 15 mins in a methylene blue solution the cells start to die, so you have to do the tests in parallel)
→ always empty the chamber and clean it
→ make sure the sample is homogeneous (shake it)
→ if you're using a thoma chamber - make sure the numbers of squares is consisent with the oculyze system - one photo taken with the oculyze system is roughly equal to one square on the thoma chamber

If you have any questions during the comparison process - please consult our team of experts at support@oculyze.de